The Centers for Disease Control and Prevention's indoor travel mask mandate was struck down by a Florida judge, and the Department of Justice said it would appeal.
The move came after the CDC asked the Biden administration to step in.
The agency said in a press release that it believes the order is within the authority of the CDC.
A notice of appeal was filed by the Justice Department on Wednesday.
On Monday, a Trump-appointed federal judge struck down the CDC's mask mandate on airplanes and other modes of public transportation.
If the CDC assessed that the mandate should remain, the Biden administration would only appeal the judge's decision. The mask mandate was extended by the CDC.
The mask requirement was dropped by several US airlines. Some passengers were informed of the policy change.
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