Freddy Rincon, a former captain of the country, died after a car crash.
The man was driving a car that collided with a bus in Cali.
Rincon played in three World Cup tournaments, scoring 17 goals for his country.
He has played in 10 World Cup games for his country, sharing the record with Carlos.
In 1990 the nation ended their 28-year wait to play at the World Cup when they qualified for the finals, and Rincon was part of the team that drew with West Germany.
He won the Club World Cup in 2000 as well as playing club football for several other teams.
The medical director of the Imbanaco Clinic in Cali where Freddy Eusebio Rincon Valencia was being treated said that he had passed away.