The league resumed in June after a three-month hiatus caused by the COVID-19 epidemic, and top-flight clubs were allowed to increase the number of subs from three to five per match.
The IFAB, the law-making body of soccer, recommended that the rule be made permanent, but the premier league decided against it because of concerns about player fatigue.
The chief executive of the Professional Footballers Association said the increasing workload on players is damaging their health and wellbeing.
The adoption of this rule is a step in the right direction to address issues with player fatigue, making sure they have the opportunity to perform at their best.
From Monday, the league will stop testing players and staff twice a week with only those who are sick to be tested.
The wellbeing of players and staff remains a priority and the Premier League will continue to monitor the national COVID-19 situation and adapt League-wide protocols as required, in line with the latest guidance from public health authorities and medical experts.
The summer transfer window will close on September 1.
Ahead of their team's game on Saturday, the managers of the two teams spoke out in favor of the rule being reintroduced.
It helps the game. Jesse Marsch told reporters that games are made faster and more intensive. I was working with five subs for two years. It makes me feel like I'm in handcuffs. It's hard to be aggressive now. Better teams have better rosters, but they also have a better XI. I like the way we play, with more fresh players on the pitch.
There is no surprise, we have spoken a lot about it. The way we play is very intense and I think five subs will be a game-changer.
It helps, definitely. There are some arguments from the small clubs that they shouldn't do it because of the sporting difference with the big clubs, but I always see it as an advantage for us.
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