The ball from Brady's last touchdown pass as a member of the Buccaneers sold for more than $500,000.
Brady threw a 55 yard touchdown pass to Mike Evans with 3:20 to go in the fourth quarter to cut the Los Angeles Rams lead to 27-20.
Evans threw the ball into the stands because Brady had not given any indication that he would retire, and there were only rumors that he was contemplating hanging it up. The fan caught the ball.
The first career touchdown pass by Brady to Terry Glenn was auctioned off on the site last year.
A total of 23 people placed bids on Brady's last touchdown ball, with the starting price of $100,000. The ball could have sold for $1 million.
Brady is still under contract with the Buccaneers and has said that he is open to a return, although coach Bruce Arians said at the NFL combine last month that he believed Brady had closed the door on returning.