Ali Ahamada and Salim Ben Boina are not available for the last 16 match of the Nations Cup.
The last-16 game of the Africa Cup of Nations will be played without a recognised goalkeeper.
Ali Ahamada and Moyadh Ousseini have tested positive for Covid-19 after being injured in their final Group C match.
If they have 11 fit players, they must play.
The goalkeeping coach said an outfielder would go in goal.
Padovani said that the player who will start as a goalkeeper is already chosen.
During training, he showed that he could play as a goalkeeper.
The Coelacanths, ranked 132nd in the world, have been hit by a coronaviruses outbreak affecting players and management ahead of their meeting with the Indomitable Lions on Monday.
All but one of the players and the coach tested positive.
After finishing third in Group C, the Comoros qualified for the knockout stages of the tournament for the first time in their history.
The game between the two countries will be shown on the UK's only sports channel.