Mick finished his course of treatment before Christmas.
Mick Harford will return to work for the FA Cup tie against Harrogate after undergoing treatment for cancer.
The 62-year-old has had a course of radiation after being diagnosed with a condition.
He told the club website that he was happy to back in full-time.
Nathan Jones will be joined by Alan Sheehan, a former captain of the team.
During his playing career, Harford made more than 200 appearances for Luton, including the 1988 League Cup final win.
He was the team's manager in 2008 and had two periods as the interim boss.
He has been given the all-clear to resume his day-to-day role after the staff at the Championship club raised more than $27,000 to help with his cancer treatment.
I have to keep on with my appointments at the hospital, but it's great to come back into a positive environment. He said it makes him feel better and he is positive about moving forward.
The third round of the FA Cup is something that is important to me and I'm looking forward to the challenge. You can get stuck into your work if you take your mind off things. I can focus on the football.
The support I've had from supporters, the club, the board, the management, staff and players has been amazing. I can't thank them enough.
Jones said everyone was delighted to have Harford back at the club and that he had made an "instant impact" in training this week.
He's a big part of the culture and environment. Jones said that he and Mick always talked about when he would return.
He feels that now is the right time after a few days of keeping his hand in.
