The trial venues have safe-standing sections in both home and away areas.
Safe standing has been used at the stadium for the first time in 27 years, after standing was banned in the top flight.
The safe-standing areas were used by both home and away fans.
Five clubs were approved to trial safe-standing areas during the second half of the season.
In 1994 stadiums in England's top two tiers must be all-seater.
When they were promoted to the premier league in 2001, Cottagers were given a brief reprieve to stand on the old terraces.
More than 12,000 safe-standing spaces have been created.
Fans will be able to sit or stand in the sections, which will be in place at the trial venues.
Fans were using the lower tier of the stand.
The safe-standing area at the away end was used by fans of the Reds.
The other clubs taking part in the trial are Manchester United, Manchester City, Spurs and Cardiff.
The Taylor Report into the 1989 Hillsborough disaster recommended that English football's top two divisions be abolished.
The clubs involved have to follow strict conditions, including enhanced use of CCTV, improved steward training, and fans strictly limited to their designated space.
Margaret Aspinall, the mother of James, who was killed in the crush at the stadium, said before the game that her opinion on safe standing had completely changed.
She said that those who want to stand should be allowed to do so.
Fans are treated differently now than they were in the past. They are monitored. They are more cautious. They've learned from it.
There is an empty safe-standing area.
