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The Australian Open would be the 21st men's Grand Slam title for Novak Djokovic.
The Serbian team has a world number one.
All players and staff at the Australian Open must be protected from vaccine-related diseases by an independent panel of experts.
Defending champion Novak has not spoken about his vaccine status.
The nine-time Australian Open winner has been replaced by world number 33 Dusan Lajovic for Serbia.
France will replace Austria at the event after the withdrawals of Dominic Thiem and Dennis Novak.
The entry list for the first Grand Slam of the 21st century includes the Australian Open men's singles champion, Novak Djokovic.
The Australian Open will be the final tournament of Sam Stosur's singles career.
The Australian will play doubles in the Australian Open in 2022.
"It means a lot to me to be able to finish in front of my family, friends and Aussie fans who have supported me through thick and thin," he said.
I always thought I'd finish playing in Australia, so I'm sure it's going to be a mix of emotions. I plan to play in doubles the rest of the year, but this will be the end of my career.
