Mason Mount will be expected to make a comeback to England's line-up against Ukraine on Saturday, but Bukayo Saka remains doubtful after suffering a knock during training Friday.
Mount was placed in precautionary isolation along with Ben Chilwell for more than a week after close contact with Billy Gilmour (club team-mate), who tested positive for Covid-19 after the goalless Group D draw against Scotland at Wembley.
Mount was released from quarantine on Monday at midnight and had been training with the rest of his squad since Wednesday. He is now ready to go to Gareth Southgate's side for the return trip to Rome.
Image: Bukayo Saka was injured in training on Friday
Saka, however, will have to pass a fitness test before Saturday's match.
England boss Gareth Southgate stated, "We just have to check on Bukayo who had a slight knock today"
"Other then that, everybody is available."
If Southgate chooses his strongest team, Jordan Pickford and Kyle Walker will all be in Southgate's starting lineup. Kieran Trippier is suffering from a minor hamstring injury that must be treated.
If Saka is unable to play, Jadon Sancho may start the first game of tournament. Phil Foden could return to England's right-hand side after he was withdrawn from the final group match against the Czech Republic.
1:30 Gareth Southgate suggests that playing Phil Foden or Jack Grealish against Ukraine could be an option
Foden and Jack Grealish are yet to appear together in the tournament, but Southgate said that it was not due to a clash between styles.
He said, "Absolutely they are able to work together." They have played together in the past. We have such an array of attacking talent.
"I have always said it is impossible to please everyone, and to choose a team that everyone will like. We have to choose the right players at the right times, to see what is going to cause the opposition the most trouble, where the players are physically, and when they can make an impact.
Stephen Warnock says that 0:36 Grealish is the right time to begin for an attack-minded England against Ukraine
"They have been so respectful of each other and this is a testament to our strength as a team.
"Raheem (Sterling), has been on fire. It has been more difficult for others to join that left side because of his goals."
Southgate would like to play with a back four again against Ukraine, but the decision about formation is more difficult than it was against Germany because England knew that their opponents would be playing 3-4-3.
However, the formation of Ukraine is much more uncertain. They were 4-3-3 in group games, but switched to 3-5-2 after the knockout victory against Sweden.
0:50 Southgate states that he hasn't considered resting players on a yellow and believes England owes it to Ukraine to play the strongest side.
Southgate stated that it would be an insult to Ukraine to plan beyond Saturday’s quarter-final and rest the four England players, who are one booking away from missing a possible last-four date.
If they are cautioned at StadioOlimpico, Harry Maguire, Kalvin Philips, Declan Rice, and Foden are suspended from a possible semi-final match against Denmark or the Czech Republic.
After the quarter-final stage, all bookings have been wiped and Jose Mourinho, Roma manager, said that the quartet should rest against Ukraine on Saturday in order to avoid suspension for the semi final.
Southgate left Foden out the squad for the last group stage game, a 1-0 victory over the Czech Republic, to avoid a ban and said that he did not intend to follow suit against Ukraine.
Image: Foden is not in the picture since he was substituted during the goalless draw with Scotland
"I can understand why you might want to do it strategically, but I cannot think of any country that would do that in a quarter-final. Not a country that has been to just three semi-finals throughout its history.
"I believe we should be focusing on tomorrow. It would be a huge error to think about other things, and it would also be insulting to Ukraine.
Image: Harry Maguire is just one booking away, but he says it won't stop him from playing his regular game
Maguire disagrees with the UEFA regulations, but insists it will not stop him from playing against Andriy Shevchenko's surprise quarterfinalists.
He said that although two yellow cards in five games seemed a little excessive for the penalty of missing a semi final, that was the rule and what we must deal with.
"But for me I'll just go to the game as usual. You don't often play in quarter-finals for your country, and we haven't been there as many times in the past.
"So, the chance is there to perform and do all I can to reach semi-final. Then we'll be able to think about it when it happens.
"At the moment, I am only fully focused on the quarterfinal and that is what I'll think about. I'll just play a normal match."
1:55 Maguire said he was happy to play in a back 3 or 4 and that modern football requires teams to be fluid in their set-ups
Oleksandr Ziinchenko believes that Ukraine must play the best game of their lives to win against England.
Zinchenko, a versatile Manchester City player, is the most prominent name in the Ukrainian team and knows that his team will face a tough battle against familiar faces at Stadio Olimpico.
Zinchenko stated that England's numbers speak for themselves. "The fact that England has not conceded any goals yet is a sign they play well in defense.
"But I believe that there are not perfect teams. Each team has its weaknesses.
0:45 Oleksandr Ziinchenko has praised Manchester City's team-mates Kyle Walker, John Stones and said that Ukraine must play the 'best game of their lives' in order to defeat England
Henderson: It is delusional to underestimate Ukraine
Jordan Henderson, vice-captain of England, says that anyone who underestimates quarter-final opponents Ukraine are "delusional".
England are the clear favourites after beating Germany 2-0 at Wembley on Tuesday's last-16 match. However, Henderson isn't taking anything for granted going into the quarter-final.
0:52 Ex England striker Emile Heskey analyzes the balance Southgate must find between defensive strength and attack in the Euro 2020 quarterfinal against Ukraine
The 31-year old said, "It was an amazing performance but we haven’t achieved anything yet and still have a long way left to go."
"Pull attention on Ukraine. It will be a very difficult test, and anyone who says it won't is delusional.
"I know that people will say, "Well, we beat Germany", but it is important to remember the significance of this game. You can't underestimate how good Ukraine are and how difficult it will be against them.
"We are fully prepared and aware of this. It's up to us to give everything and continue to do so.
England is now the favourite to win Euro 2020 after beating Germany. Jamie Carragher was able to give his verdict on England's historic victory over Germany when he joined Sky Sports Football Euros Podcast. He discusses why Gareth Southgate's team could reach new heights, and also evaluates the squad's key players and how the manager might shake things up for the quarter-final match with Ukraine.
Part two of the show features Peter Smith and Sky Sports' football writer Charlotte Marsh, who join Adam Smith, Sky Sports data editor Adam Smith, to discuss the other quarter-finals. They also work out who will advance from the ties involving Spain vs Spain and Italy vs Italy.
With their victories over Switzerland and Belgium on Friday, Spain and Italy booked semi-final dates at Wembley. It's now on to the Czech Republic, Denmark and Ukraine.
Sky Sports' Peter Smith and Adam Bate join Kate Burlaga to analyze the first quarter-finals. The panel also discusses the strengths and weaknesses of Spain, as well as what makes Italy an effective opponent.
The guests also look ahead to England's Saturday matchup with Ukraine in Rome. They weigh up Gareth Southgate's team selection dilemmas and determine where the big game will end.