Bob Yirka is a research scientist at Phys.org.

Some archaea found to have integrons, allowing cross-domain gene transfer
Cassette recruitment (attC × attI recombination) assays. (A) Schematic outlining the experimental setup of the cassette insertion assays. The kanamycin resistance (KmR) suicide vector pJP5603 with an attC site is delivered into the recipient E. coli UB5201 strain via conjugation. The recipient strain carries an intI1 gene, expressed from the inducible PBAD promoter, and an attI1 site, residing on the carbenicillin resistance (CbR) pBAD24 and chloramphenicol resistance (CmR) pACYC184 backbones, respectively. The donor suicide vector cannot replicate within the recipient host and thus can only persist following attC × attI recombination to form a plasmid cointegrate. (B) Average recombination frequencies (log10 scale, ±1 SE) between attI1 and nine archaeal attCs (with phyla of origin labeled along the x axis) and the paradigmatic bacterial attC site (attCaadA7), used as positive control. Average frequencies were calculated following three independent cassette insertion assays (see Materials and Methods for details). No statistically significant difference in recombination frequencies were detected among the tested attCs (Kruskal-Wallis test, n = 27; df = 8, P = 0.488). Recombination frequencies are shown for attC bottom strands only. See table S1 for attC top strand recombination frequencies. N.S., not significant. Credit: Science Advances (2022). DOI: 10.1126/sciadv.abq6376

Evidence shows that some Archaea have integrons. In their paper published in the journal Science Advances, the group describes how they used a recently developed technique called metagenome-assembled genomes to study the genomes of Archaea samples.

The life on Earth is divided into three different areas. The third domain is called Archaea. Like Bacteria, Archaea are single-celled, but they rely on lipids in their cells.

In this new effort, the researchers were looking into the ways in whichbacteria swap genes and wondered if it was possible that they have integrons. To find out, they used a technique that allows for searching for single genes and genes that are related.

They used the technique to find lots of matches. 75 matches of an integron were found in the 6,700 genomes scanned. The structure of the integrons inbacteria suggested the use of cassettes.

The researchers believed that this showed that the Archaea should be able to swap genes with other organisms. They exposed AttC to an E. coli specimen in order to prove their idea was correct. The cassettes had been created to allow the swap of genes.

New avenues of research will be opened up when integrons are found. One way to look into the possibility is to swap genes from Archaea tobacteria in order to make them resistant to drugs. A complete genome of Archaea would be helpful.

There is more information about the Discovery of integrons in Archaea: Platforms for cross-domain Gene Transfer. There is a book titled "Sci Adv.abq6376."

Journal information: Science Advances

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