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The doctor for the Brazil team says that the player will not be able to play in the next two World Cup games due to injury.
A swollen ankle was shown in pictures as he sat with his face covered.
The right-back will be out for the next two games.
Brazil will play Switzerland on Monday in their second game of the group.
The doctor for the Brazil team said that they found damage to the ankle of two of their players.
Being calm and evaluating day by day is important. For the rest of the competition, the idea is to get them back.
Speaking after the win against Serbia, Brazil head coach Tite said they were confident that Neymar would play in the World Cup and that he didn't see he was injured.
Over the past few years, the Paris St-Germain forward has had a number of injuries to his foot.
He missed the Copa America due to an ankle injury and was out for several weeks in 2021, due to a different ankle injury.
The Brazilian player drew four more free-kicks than any other player at the World Cup so far, as a result of being foul nine times in the game against Serbia.
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