Nancy Pelosi's husband was attacked with a hammer by a man who was trying to abduct the speaker.
At a rally near Dayton, Ohio, on behalf of Republican candidates, Trump referred to Pelosi and the House impeaching him twice.
The comment about Pelosi was made by Trump immediately after he mentioned the gang member who was convicted of murder.
The speaker's husband, Paul Pelosi, was attacked in San Francisco by a person accused of trying to abduct her. Democrats are concerned about political violence and Republican rhetoric.
"They will say, 'What a horrible thing,'" Trump said. The former president claimed that he called Nancy Pelosi an animal.
The word bullshit will never be used again by me. At the rally, Trump said, "But what she did to us in this country?"
He referred to the speaker as crazy many times before.