The England left-back's World Cup dream is over after a Scan showed he has a serious injury.
The injury to Chilwell occurred in the Blues' victory over Dinamo Zagreb.
He was in England's most recent squad but did not play.
England will play Iran in the World Cup on November 21st.
He suffered a knee injury in November and was out for a long time.
"Following my ACL injury I worked so hard to make sure I was ready for the World Cup," Chilwell wrote on social media.
It's been a dream of mine for a long time. This will not be possible after my scans. I'll do everything I can to get back in the lineup for the Blues.
He was in the England squad for Euro 2020 but did not play.
He is expected to miss the World Cup due to a serious injury.
They said that Ben would start a rehabilitation programme with the club's medical department.
As England prepare for the tournament, they have lost another defender to injury.
Kyle Walker is confident that he will make the squad despite undergoing groin surgery, even though he is a doubt with a knee injury.
Kyle Walker-Peters is going to be out for the rest of the season with a injury.
KalvinPhillips is a doubt for the tournament after dislocating his shoulder, butPep isoptimistic that he will be fit in time for the tournament.
The most recent game for England was against Germany and the left wing-back was Manchester United'sLuke Shaw.