Sources told Adam Schefter that the Los Angeles Clippers left tackle will be out for the rest of the season with a torn bicep.
After sustaining an injury in the third quarter, he was replaced by StormNorton.
The loss of the Pro Bowl center, who is dealing with a knee issue, is a big blow to the team.
He was the 13th overall pick in the draft and earned Pro Bowl recognition as a first year player.
After opening the season with a win over the Las Vegas Raiders, the Bolts have lost their next two games against the Kansas City Chiefs and Jacksonville.
Herbert has a fractured rib and is expected to be out for a long time.
In the past two games, receiver Keenan Allen and receiver Jalen Guyton have been injured.
There is uncertainty about the severity of Joey Bosa's groin injury, which he sustained in the first quarter of the game.
The status of Jackson, who signed a five-year, $82.50 million contract in free agency, remains unclear. Jackson missed the first week after having ankle surgery. He was on the inactive list for the game against the Jags.