The firm will have to pay more than $4 billion to the EU after judges rejected their appeal.
The tech giant was fined by Europe's competition watchdog for abusing its dominance in the mobile operating system.
In the last three years, the EU has fined the tech giant over claims of anticompetitive behavior.
The decision comes almost a year after the company lost an appeal against the EU fine for promoting its own shopping service in its search results.
Margrethe Vestager, the European Union's competition commissioner, has a reputation for being Silicon Valley's policewoman.
The story is getting better.