Scientists can look at the expression of genes in a cell. The activity of thousands of genes in these cells can be measured with the help of information from the original genes.
Most cells exist in a mixed population, which makes it difficult to capture a specific cell from a specific population. Scientists have developed "scRNA-seq," which provides unprecedented insights into basic and medical research.
Live-seq is a new product.
The problem of needing to lyse cells has been solved by the scientists from the group of professors. Dr. Wanze Chen and Dr. Orane Guillaume Gentil are part of the research team. The innovative approach keeps the cells alive during the research process.
The key to Live-seq is a technique called fluidic force microscopy, which uses tiny channels to manipulate fluids under a microscope. It is possible to insert substances into individual cells or to extract cytoplasm from single cells without having to kill them with a gun.
The advance was made when the researchers were able to read out the transcriptome from the small amount of sample. Live-seq can now connect a cell's transcriptome at a given time to its later behavior.
The researchers found that Live-seq can accurately identify diverse cell types and states. As a proof-of-concept, they used their new platform to map thejectory of individual immune cells before and after they became active. The team was able to predict how strong an immune cell would be by using Live-seq.
The work is published in a journal. Live-seq can address a broad range of biological questions by transforming scRNA-seq from an end point to a temporal and spatial analysis approach.
More information: Bart Deplancke, Live-seq enables temporal transcriptomic recording of single cells, Nature (2022). DOI: 10.1038/s41586-022-05046-9. www.nature.com/articles/s41586-022-05046-9 Journal information: Nature
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