Mass spectrometry-based draft of the mouse proteome

Almost every aspect of life is controlled and organized by genes. The proteome is the totality of all the genes in a living creature. The proteome is the complement of the genome in model organisms. A draft human proteome was reported for the first time by a team at the Chair of Proteomics and Bioanalytics.

The laboratory mouse is one of the most important experimental systems for the study of diseases. A draft of the mouse proteome is presented. "These molecule are cellular regulators of normal as well as pathological processes," says Professor Kster.

Humans, animals and plants have similar proteomes.

Insights can be gleaned from the data according to the researchers. For example, tissue- and cell-type resolved profiles give evidence for the expression of 17,000 genes and thousands of isoforms, which are different from one another.

There are 50,000 sites that could be identified. These are parts of the cell's machinery. In other words, the research work provides information about how many mouse genes exist and how active they are.

There are many similarities between mouse, human and Arabidopsis, but many differentially abundant orthologs that serve species specific functions. The proteome of the mouse is used to integrate more than 400 drug and radiation response data sets with the proteomes of 66 PDAC cell lines.

There are other resources for the mouse. The information can be found in the online databases.

More information: Piero Giansanti et al, Mass spectrometry-based draft of the mouse proteome, Nature Methods (2022). DOI: 10.1038/s41592-022-01526-y Journal information: Nature Methods