The Florida Supreme Court turned down a request for a hearing over the state's controversial new congressional map that eliminates a majority Black district.
The groups want the court to consider reinstating the injunction.
The plan to move the 5th Congressional District out of a majority Black area of north Florida was found to violate the Fair Districts Amendments of the state constitution.
The court did not rule on the merits of the lawsuit but its decision to not issue an injunction effectively kills any chance of changing the map before the elections.
The map was drawn by the governor's office before it was approved by the legislature. Black residents make up 40% of the population in the 5th district, which was straddled by the old map along the Florida-Georgia border. The shape of the district made it unconstitutional, while critics said it was a move to diminish the Black vote.
There is a new item on the market. The number of Republican-leaning districts in Florida has increased by four over the previous map.
Major court challenges have favored Republicans. In New York, a court blocked the state from implementing a new map approved by the Democrats.
A judge in Florida has blocked a map that would affect black voters.
The Florida Congressional Map Eliminating Black District was thrown out by the court.