Two people in London have been diagnosed with a disease.
The UK Health Security Agency said that the two people living in the same household are not related to the previous confirmed case in England.
One person is receiving care at the infectious disease unit at St Mary's hospital, which is part of the Imperial College healthcare trust in London.
The other does not need hospital treatment, according to the UKHSA.
Health officials are looking into how the infections were acquired.
The agency said people who might have been in close contact with either person were being contacted and given information and health advice.
The director of clinical and emerging infections at the UKHSA, Dr Colin Brown, said that there are two new monkeypox cases in England that are unrelated to the case announced on May 7.
It is important to point out that it does not spread easily between people and requires close personal contact with an affected person. The risk to the general public is very low.
We are reaching out to potential friends, family and contacts in the community. We are trying to get in touch with any healthcare contacts who have had close contact with the cases prior to confirmation of their infections, to assess them and give advice.