Giorgio Chiellini, who has been with the club for 17 years, will leave at the end of the season.
Since joining the club in 2005, the centre-back has won nine Serie A titles, as well as the Coppa Italia five times.
The Italian Cup final was won by Inter Milan.
Speaking after the 4-2 defeat in Rome, Chiellini said: "I hope I have left something behind."
After winning his first title with the club, Chiellini went on to win nine in a row with Inter.
He told Mediaset that it was up to the others to continue.
I will say goodbye to my stadium on Monday and if I don't have anything left in the tank, I might have a run-out in Florence. It is my decision.
Massimiliano Allegri's side are assured of a top-four finish with two league games remaining, and host Lazio on Monday.
The stadium where Italy beat England to win the Euro 2020 final last summer is where Chiellini will retire after Italy play Argentina in June.
He is the fifth most-capped player in Italy, alongside the likes ofAndrea Pirlo.
He doesn't know if he will continue to play at club level.
Chiellini said that he needed to think about it with his family.
I am happy to leave at such a high level because for many years I have said I didn't want to finish struggling and not be able to play at my level.
I gave it my all. I will be the biggest fan from the outside. You can't shake it off after so many years in this club.